• Issue
    Volume 6, Issue 2
    i-iv, 41-71
    February 2024


Free Access

Issue Information

  • Pages: i-iv
  • First Published: 08 February 2024


Open Access

Effect of air–liquid interface on cultured human intestinal epithelial cells

  • Pages: 41-52
  • First Published: 21 December 2023
Description unavailable

Small intestinal tissue samples were obtained to form enteroids in a transwell culture system. The transwell system was cultured either under submerged or ALI conditions. We found that the human intestinal monolayer exhibited similar morphology as in vivo. The brush border formation was observed apically in the ALI conditions. Tight junction was also preserved in the ALI conditions. The ALI conditions positively contribute toward a differentiated phenotype of epithelial cells.