Psoralen accelerates bone fracture healing by activating both osteoclasts and osteoblasts
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- Volume 34Issue 9The FASEB Journal
- pages: 13069-13069
- First Published online: July 23, 2020
Tan Zhang
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
These authors contributed equally to this work.Search for more papers by this authorWeiqi Han
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
These authors contributed equally to this work.Search for more papers by this authorKangxian Zhao
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
Search for more papers by this authorWanlei Yang
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
Search for more papers by this authorXuanyuan Lu
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
Search for more papers by this authorYewei Jia
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
Search for more papers by this authorAn Qin
Department of Orthopedics, Shanghai Key Laboratory of Orthopedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China
Search for more papers by this authorCorresponding Author
Yu Qian
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
Correspondence: Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, #568 Zhongxing North Rd., Shaoxing, Zhejiang 312000, China. E-mail: [email protected]
Search for more papers by this authorTan Zhang
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
These authors contributed equally to this work.Search for more papers by this authorWeiqi Han
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
These authors contributed equally to this work.Search for more papers by this authorKangxian Zhao
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
Search for more papers by this authorWanlei Yang
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
Search for more papers by this authorXuanyuan Lu
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
Search for more papers by this authorYewei Jia
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
Search for more papers by this authorAn Qin
Department of Orthopedics, Shanghai Key Laboratory of Orthopedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China
Search for more papers by this authorCorresponding Author
Yu Qian
Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, Shaoxing, China
Correspondence: Department of Orthopaedics, Shaoxing People's Hospital, Zhejiang University School of Medicine, #568 Zhongxing North Rd., Shaoxing, Zhejiang 312000, China. E-mail: [email protected]
Search for more papers by this authorABSTRACT
Bone fracture healing is a complex, dynamic process that involves various cell types, with osteoclasts and osteoblasts playing indispensable roles. In this study, we found that psoralen, the main active ingredient in Psoralea corylifolia L. fruit extract, enhanced bone fracture healing through activation of osteoclast and osteoblast activity via the ERK signaling pathway. In detail, psoralen promoted receptor activator of nuclear factor-κB ligand-induced osteoclastogenesis, mRNA expression of osteoclast-specific genes, and osteoclastic bone resorption in primary bone marrow-derived macrophages. Meanwhile, psoralen induced osteogenic differentiation by promoting the mRNA expression of the osteoblast differentiation markers alkaline phosphatase, runt-related transcription factor 2, osterix, and osteocalcin. At the molecular level, psoralen preferentially activated ERK1/2 but not JNK or p38 MAPKs. Further experiments revealed that psoralen-induced osteoclast and osteoblast differentiation was abrogated by a specific inhibitor of phosphorylated ERK. In addition, psoralen accelerated bone fracture healing in a rat tibial fracture model, and the numbers of osteoclasts and osteoblasts were increased in psoralen-treated fracture callus. Taken together, our findings indicate that psoralen accelerates bone fracture healing through activation of osteoclasts and osteoblasts via ERK signaling and has potential as a novel drug in the orthopedic clinic for the treatment of bone fractures.—Zhang, T., Han, W., Zhao, K., Yang, W., Lu, X., Jia, Y., Qin, A., Qian, Y. Psoralen accelerates bone fracture healing by activating both osteoclasts and osteoblasts. FASEB J. 33, 5399–5410 (2019). www.fasebj.org
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