Engineering an in situ crosslinkable hydrogel for enhanced remyelination
Xiaowei Li
Clemson–Medical University of South Carolina Bioengineering Program, Department of Bioengineering, Clemson University, Charleston, South Carolina, USA
Search for more papers by this authorXiaoyan Liu
Clemson–Medical University of South Carolina Bioengineering Program, Department of Bioengineering, Clemson University, Charleston, South Carolina, USA
Search for more papers by this authorLin Cui
Clemson–Medical University of South Carolina Bioengineering Program, Department of Bioengineering, Clemson University, Charleston, South Carolina, USA
Search for more papers by this authorChristopher Brunson
Department of Chemical Engineering, Stanford University, Stanford, California, USA
Search for more papers by this authorWen Zhao
Clemson–Medical University of South Carolina Bioengineering Program, Department of Bioengineering, Clemson University, Charleston, South Carolina, USA
Department of Orthopedic Surgery, Beijing Aerospace General Hospital, Beijing, China
Search for more papers by this authorNarayan R. Bhat
Institute of Neuroscience, Medical University of South Carolina, Charleston, South Carolina, USA
Search for more papers by this authorCorresponding Author
Ning Zhang
Clemson–Medical University of South Carolina Bioengineering Program, Department of Bioengineering, Clemson University, Charleston, South Carolina, USA
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina, USA
Correspondence: Clemson-MUSC Bioengineering Program, Department of Bioengineering, Clemson University, 68 President St., BEB 313, Charleston, SC, 29425, USA. E-mail: N.Z., [email protected]; X.W., [email protected]Search for more papers by this authorCorresponding Author
Xuejun Wen
Clemson–Medical University of South Carolina Bioengineering Program, Department of Bioengineering, Clemson University, Charleston, South Carolina, USA
Institute of Neuroscience, Medical University of South Carolina, Charleston, South Carolina, USA
Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, South Carolina, USA
Department of Orthopedic Surgery, Medical University of South Carolina, Charleston, South Carolina, USA
Institute for Advanced Materials and Nano Biomedicine (iNANO), Tongji University, Shanghai, China
Correspondence: Clemson-MUSC Bioengineering Program, Department of Bioengineering, Clemson University, 68 President St., BEB 313, Charleston, SC, 29425, USA. E-mail: N.Z., [email protected]; X.W., [email protected]Search for more papers by this authorXiaowei Li
Clemson–Medical University of South Carolina Bioengineering Program, Department of Bioengineering, Clemson University, Charleston, South Carolina, USA
Search for more papers by this authorXiaoyan Liu
Clemson–Medical University of South Carolina Bioengineering Program, Department of Bioengineering, Clemson University, Charleston, South Carolina, USA
Search for more papers by this authorLin Cui
Clemson–Medical University of South Carolina Bioengineering Program, Department of Bioengineering, Clemson University, Charleston, South Carolina, USA
Search for more papers by this authorChristopher Brunson
Department of Chemical Engineering, Stanford University, Stanford, California, USA
Search for more papers by this authorWen Zhao
Clemson–Medical University of South Carolina Bioengineering Program, Department of Bioengineering, Clemson University, Charleston, South Carolina, USA
Department of Orthopedic Surgery, Beijing Aerospace General Hospital, Beijing, China
Search for more papers by this authorNarayan R. Bhat
Institute of Neuroscience, Medical University of South Carolina, Charleston, South Carolina, USA
Search for more papers by this authorCorresponding Author
Ning Zhang
Clemson–Medical University of South Carolina Bioengineering Program, Department of Bioengineering, Clemson University, Charleston, South Carolina, USA
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina, USA
Correspondence: Clemson-MUSC Bioengineering Program, Department of Bioengineering, Clemson University, 68 President St., BEB 313, Charleston, SC, 29425, USA. E-mail: N.Z., [email protected]; X.W., [email protected]Search for more papers by this authorCorresponding Author
Xuejun Wen
Clemson–Medical University of South Carolina Bioengineering Program, Department of Bioengineering, Clemson University, Charleston, South Carolina, USA
Institute of Neuroscience, Medical University of South Carolina, Charleston, South Carolina, USA
Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, South Carolina, USA
Department of Orthopedic Surgery, Medical University of South Carolina, Charleston, South Carolina, USA
Institute for Advanced Materials and Nano Biomedicine (iNANO), Tongji University, Shanghai, China
Correspondence: Clemson-MUSC Bioengineering Program, Department of Bioengineering, Clemson University, 68 President St., BEB 313, Charleston, SC, 29425, USA. E-mail: N.Z., [email protected]; X.W., [email protected]Search for more papers by this authorAbstract
Remyelination has to occur to fully regenerate injured spinal cords or brain tissues. A growing body of evidence has suggested that exogenous cell transplantation is one promising strategy to promote remyelination. However, direct injection of neural stem cells or oligodendrocyte progenitor cells (OPCs) to the lesion site may not be an optimal therapeutic strategy due to poor viability and functionality of transplanted cells resulted from the local hostile tissue environment. The overall objective of this study was to engineer an injectable biocompatible hydrogel system as a supportive niche to provide a regeneration permissive microenvironment for transplanted OPCs to survive, functionally differentiate, and remyelinate central nervous system (CNS) lesions. A highly biocompatible hydrogel, based on thiol-functionalized hyaluronic acid and thiol-functionalized gelatin, which can be crosslinked by poly-(ethylene glycol) diacrylate (PEGDA), was used. These hydrogels were optimized first regarding cell adhesive properties and mechanical properties to best support the growth properties of OPCs in culture. Transplanted OPCs with the hydrogels optimized in vitro exhibited enhanced survival and oligodendrogenic differentiation and were able to remyelinate demyelinated axons inside ethidium bromide (EB) demyelination lesion in adult spinal cord. This study provides a new possible therapeutic approach to treat CNS injuries in which cell therapies may be essential.—Li, X., Liu, X., Cui, L., Brunson, C., Zhao, W., Bhat, N. R., Zhang, N., Wen, X. Engineering an in situ crosslinkable hydrogel for enhanced remyelination. FASEB J. 27, 1127–1136 (2013). www.fasebj.org
Supporting Information
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| fsb2027003027-sup-0001.zipZip archive, 3.5 MB | Supplementary material |
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