Volume 14, Issue 10 p. 1345-1351
Research Communication
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Natriuretic peptides: a new lipolytic pathway in human adipocytes

Coralie Sengenès

Coralie Sengenès

INSERM U 317 and Laboratoire de Pharmacologie Médicale et Clinique, Faculté de Médecine, Toulouse Cedex, 31073 France

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Michel Berlan

Michel Berlan

INSERM U 317 and Laboratoire de Pharmacologie Médicale et Clinique, Faculté de Médecine, Toulouse Cedex, 31073 France

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Isabelle de Glisezinski

Isabelle de Glisezinski

INSERM U 317 and Laboratoire de Pharmacologie Médicale et Clinique, Faculté de Médecine, Toulouse Cedex, 31073 France

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Max Lafontan

Max Lafontan

INSERM U 317 and Laboratoire de Pharmacologie Médicale et Clinique, Faculté de Médecine, Toulouse Cedex, 31073 France

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Jean Galitzky

Corresponding Author

Jean Galitzky

INSERM U 317 and Laboratoire de Pharmacologie Médicale et Clinique, Faculté de Médecine, Toulouse Cedex, 31073 France

Correspondence: INSERM U317, Laboratoire de Pharmacologie Médicale et Clinique, Faculté de Médecine, 37 Allées Jules Guesde, 31073 Toulouse Cedex, France. E-mail: [email protected]Search for more papers by this author
First published: 01 July 2000
Citations: 218

Abstract

Atrial natriuretic peptide (ANP) receptors have been described on rodent adipocytes and expression of their mRNA is found in human adipose tissue. However, no biological effects associated with the stimulation of these receptors have been reported in this tissue. A putative lipolytic effect of natriuretic peptides was investigated in human adipose tissue. On isolated fat cells, ANP and brain natriuretic peptide (BNP) stimulated lipolysis as much as isoproterenol, a nonselective β-adrenergic receptor agonist, whereas C-type natriuretic peptide (CNP) had the lowest lipolytic effect. In situ microdialysis experiments confirmed the potent lipolytic effect of ANP in abdominal s.c. adipose tissue of healthy subjects. A high level of ANP binding sites was identified in human adipocytes. The potency order defined in lipolysis (ANP > BNP > CNP) and the ANP-induced cGMP production sustained the presence of type A natriuretic peptide receptor in human fat cells. Activation or inhibition of cGMP-inhibited phosphodiesterase (PDE-3B) (using insulin and OPC 3911, respectively) did not modify ANP-induced lipolysis whereas the isoproterenol effect was decreased or increased. Moreover, inhibition of adenylyl cyclase activity (using a mixture of α2-adrenergic and adenosine A1 agonists receptors) did not change ANP- but suppressed isoproterenol-induced lipolysis. The noninvolvement of the PDE-3B was finally confirmed by measuring its activity under ANP stimulation. Thus, we demonstrate that natriuretic pep tides are a new pathway controlling human adipose tissue lipolysis operating via a cGMP-dependent pathway that does not involve PDE-3B inhibition and cAMP production.—Sengenès, C., Berlan, M., De Glisezinski, I., Lafontan, M., Galitzky, J. Natriuretic peptides: a new lipolytic pathway in human adipocytes. FASEB J. 14, 1345–1351 (2000)